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Starting from day 6 we included 10 M cyclopamine to promote dorsalization

This age-related decline has made it a subject of considerable interest in longevity and tissue repair research

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Physiol Plant 100:234240 Foreman J, Demidchik V, Bothwell JH, Mylona P, Miedema H, Torres MA, Linstead P, Costa S, Brownlee C, Jones JD, Davies JM, Dolan L (2003) Reactive oxygen species produced by NADPH oxidase regulate plant cell growth

These agents should not be used if fever, bloody stools, or severe abdominal pain are present, as these symptoms may indicate a more serious condition requiring medical evaluation